Measuring How a Sample Interacts With Light
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Chemistry Expert
- Ph.D., Biomedical Sciences, University of Tennessee at Knoxville
- B.A., Physics and Mathematics, Hastings College
Dr. Helmenstine holds a Ph.D. in biomedical sciences and is a science writer, educator, and consultant. She has taught science courses at the high school, college, and graduate levels.
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Updated on August 02, 2022
Absorbance is a measure of the quantity of light absorbed by a sample. It is also known as optical density, extinction, or decadic absorbance. The property is measured using spectroscopy, particularly for quantitative analysis. Typical units of absorbance are called "absorbance units," which have the abbreviation AU and are dimensionless.
Absorbance is calculated based on either the amount of light reflected or scattered by a sample or by the amount transmitted through a sample. If all light passes through a sample, none was absorbed, so the absorbance is zero and the transmission is 100%. On the other hand, if no light passes through a sample, the absorbance is infinite and the percent transmission is zero.
The Beer-Lambert law is used to calculate absorbance:
A = ebc
Where A is absorbance (no units, A = log10P0/ P)
eis the molar absorptivity with units of L mol-1cm-1
bis the path length of the sample, usually the length of a cuvette in centimeters
cis the concentration of a solute in solution, expressed in mol/L
Sources
- IUPAC (1997). Compendium of Chemical Terminology, 2nd ed. (the "Gold Book").
- Zitzewitz, Paul W. (1999). Glencoe Physics. New York, N.Y.: Glencoe/McGraw-Hill. p. 395. ISBN 0-02-825473-2.
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Helmenstine, Anne Marie, Ph.D. "Absorbance Definition in Chemistry." ThoughtCo, Aug. 2, 2022, thoughtco.com/definition-of-absorbance-604351.Helmenstine, Anne Marie, Ph.D. (2022, August 2). Absorbance Definition in Chemistry. Retrieved from https://www.thoughtco.com/definition-of-absorbance-604351Helmenstine, Anne Marie, Ph.D. "Absorbance Definition in Chemistry." ThoughtCo. https://www.thoughtco.com/definition-of-absorbance-604351 (accessed June 7, 2024).
